Application

Anti-Monocyte Chemotactic Protein-1 Antibody is an antibody against Monocyte Chemotactic Protein-1 for use in ELISA, WB, NEUT.

ELISA: To detect rat MCP-1 (MCAF) by direct ELISA (using 100 µL/well antibody solution) a concentration of at lease 0.5 µg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant rat MCP-1 (MCAF).

Western blot: 0.1 - 0.2 µg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat MCP-1 (100 ng/mL), a concentration of 4.0-6.0 µg/mL of this antibody is required.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly pure (>98%) recombinant rat MCP-1

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.4. Contains no preservative. Reconstitute with sterile distilled water to 0.1 - 1.0 mg/ml.

Specificity

Reacts with rat MCP-1

Storage and Stability

Maintain lyophilized material desiccated at 2°-8°:C for up to 12 months from date of receipt. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.